| LC3

Microtubule-associated protein 1 light chain 3 (MAP1LC3), commonly abbreviated as LC3, is a soluble protein with a molecular weight of approximately 17 kDa. This evolutionarily conserved protein is ubiquitously expressed in mammalian tissues and cultured cells, serving as an essential component of autophagy - the eukaryotic cellular recycling system.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.05	1.12	0.57	0.35	0.25	0.2	0.18	0.08
Corrected OD	1.97	1.04	0.49	0.27	0.17	0.12	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat LC3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	96
Cell Lysates(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Rat LC3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107